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#Qiagen qpcr free#

A simplified work flow and reduced cycling times enable screening and validation of the greatest number of samples and targets in the shortest period of time. ITaq Universal One-Step RT-qPCR Kits are a fast and convenient solution for real-time PCR using the powerful combination of iScript RNase H+ MMLV reverse transcriptase and hot-start iTaq DNA Polymerase in one reaction, providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity, and inhibitor tolerance without affecting performance.Īvailable for SYBR ®Green and probe-based qPCR assays, iTaq Universal One-Step Kits are ideal for low-to mid-throughput real-time PCR screening and validation.

#Qiagen qpcr free#

* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.Earn a free one-year service contract on your CFX System with purchase of Reagents. In addition to optimized oligo T m (based on composition, oligo length, etc.), the bioinformatics calculations address avoidance of single nucleotide polymorphisms (SNPs based on NCBI RefSeq releases) and off-target amplification, recognition of splice variants, and secondary structure predictions. These assays are designed using a proprietary algorithm. Predesigned assays are available for human, mouse, and rat targets.

MIQE compliant with all primer and probe sequences provided.

Available in 5 dye–quencher combinations and 3 reaction scales.

Guaranteed performance for all predesigned assays.

Primers and probe mixed and delivered in a single tube or 96-well plate well.

Each oligonucleotide undergoes 100% QC by mass spectrometry*, and all QC results are provided free of charge to you on the IDT website. EachĪssay is made to order with estimated shipping in 2–4 days from order receipt. XL sizes, dye–quencher combination and primer-to-probe ratio can be specified to meet unique experimental demands. Assays consist of a forward primer, a reverse primer, and a qPCR probe all delivered in a single tube or plate well. PrimeTime qPCR Probe Assays are offered in 3 different sizes to meet the needs of any qPCR experiment.

Receive primers and probe sequences with all orders.

Size and scale options available for digital PCR (dPCR) platforms.

Select a primer:probe ratio from 2:1 to 4:1 with no additional charge.

Multiplex with confidence using double-quenched ZEN ™ or TAO ™ probe assays that have the lowest background in the industry.

Select from a wide range of license-free dyes, including FAM, TET, SUN™, HEX and Cy ® 5 (Cytiva) dyes.

Achieve >90% efficiency over 4 or more orders of magnitude with our predesigned assays, guaranteed.

Create custom assays for any sequence from any species using the PrimerQuest™ Tool.

Obtain predesigned assays for human, mouse, or rat for easy selection based on multiple criteria such as exon location and number of transcriptsĭetected. PrimeTime™ qPCR Probe Assays consist of a primer pair and fluorescently labeled 5′ nuclease probe. Primer and probe premixed assays for analyzing gene expression in any species using fluorescently labeled 5′ nuclease probes Target Capture Probe Design & Ordering Tool.Order status (Genes & gene fragments only).Alt-R Predesigned Cas9 crRNA Selection Tool.